Agarose is a polysaccharide extracted from seaweed. Disappearance of supercoiled form sc, type i dna and formation of nicked circular form nc, type ii in the presence of 1 a and 2 b. Gel types there are two types of gel most typically used are agarose and polyacrylamide gels. Three principal types of gel electrophoresis are in use. In agarose gel electrophoresis, a purified, large molecular weight polysaccharide is used as the medium. It is used for immuneelectrophoresis, isoelectric focusing and electrophoresis of dna and rna in the agarose gel. Electrophoresis can be broadly divided into two types as. Starch gel electrophoresis, which makes use of potato starch granules, is something of a relic. Please use one of the following formats to cite this article in your essay, paper or report. Proteins and nucleic acids cross linked agarose gel as polyacrylamide, very large proteins, but no cross linking nucleic acids and nucleoproteins, etc. Routine protein electrophoresis performed in clinical laboratories is the oldest method and. Preparation, loading and running of gel in electrophoresis. A typical gel electrophoresis apparatus is of two kinds. Gel electrophoresis of proteins with a polyacrylamide matrix, commonly called polyacrylamide gel electrophoresis page is undoubtedly one of the most widely used techniques to characterize.
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